Unknown microbiology proteus mirabilis
The bacterium in question was tested for beta hemolysis. The orange colony was Gram stained, observed, and recorded. Bacillus subtilis is positive for starch hydrolysis pictured below on the left.
One test tube has been provided with a mixture a Gram positive and a Gram negative bacteria. For this test, I was able to finalize the result of my unknown to be Proteus vulgaris.
History of proteus vulgaris
This enzyme is secreted and hydrolyzes triglycerides to glycerol and three long chain fatty acids. This test is commonly used to distinguish between the b-hemolytic streptococci: Streptococcus agalactiae bacitracin resistant and Streptococcus pyogenes bacitracin sensitive. These enzymes break the starch molecules into smaller glucose subunits which can then enter directly into the glycolytic pathway. Escherichia coli is indole positive. The oxaloacetic acid is then hydrolyzed into pyruvic acid and CO2. KIA tubes are also capable of detecting the production of H2S. Lactose fermentation will continue to produce acidic byproducts and the media will remain yellow picture on the far left below.
Streptococcus pneumoniae and Streptococcus mitis are a-hemolytic the picture on the right below shows the a-hemolysis of S. If an organism is motile than the growth will radiate from the stab mark and make the entire tube appear turbid. Lactose fermentation will continue to produce acidic byproducts and the media will remain yellow picture on the far left below.
If gas bubbles appear, the culture is catalase positive. As a result, an arrow of beta-hemolysis is produced between the two streaks.
Is proteus mirabilis contagious
A SIM medium was used to perform the test and to detect if the bacterium was able to reduce sulfur to hydrogen sulfide H2S. The black color in the tube indicated a positive result, whereas no color change was considered negative. The organism pictured on the far left is positive for hydrogen sulfide production. TOP Oxidase Test This test is used to identify microorganisms containing the enzyme cytochrome oxidase important in the electron transport chain. Several studies have linked P. Proteus organisms are implicated as serious causes of infections in humans, along with Escherichia, Klebsiella , Enterobacter , and Serratia species. The plate below was streaked with Streptococcus pyogenes; notice the large zone of inhibition surrounding the disk. SIM tubes are inoculated with a single stab to the bottom of the tube. The Gram-positive rod, Bacillus subtilis is lipase positive pictured on the right The plate pictured on the left is lipase negative. It is seen as a black precipitate second picture from the right. The test is presumptive for S. The precipitated minerals may mix with bacteria adherent to a urinary catheter, forming a crystalline biofilm and eventually blocking urine flow through the catheter 28 , The 2,3 butanediol fermentation pathway will ferment glucose and produce a 2,3 butanediol end product instead of organic acids. When bacterium produce nitrate reductase when grown in a medium containing nitrate, the enzymes will convert the nitrate to nitrite. This is a synergistic test between Staphylococcus aureus and Streptococcus agalactiae.
Thus, hydrolysis of the starch will create a clear zone around the bacterial growth. In the urinary tract alkaline pH leads to precipitation of calcium and magnesium ions and the formation of urinary stones composed of magnesium ammonium phosphate struvite and calcium phosphate apatite
based on 103 review